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Lanczos Microscopy Resizer (ImgLib2 + ImageJ/Fiji)

A plugin designed to scale 2D and 3D image stacks plane-by-plane using high-fidelity Lanczos interpolation via the ImgLib2 framework.

Unlike standard resizing tools that break image metadata, this plugin automatically recalculates spatial calibration and embeds a digital audit trail directly into the file's properties.

🚀 How to Install and Use in Fiji / ImageJ

1. Installation

  1. Download the LanczosResizer.java file from this repository.
  2. Open Fiji / ImageJ.
  3. Drag and drop the LanczosResizer.java file directly onto the Fiji search bar, or save it into your Fiji directory under the /plugins/ folder and restart Fiji. .

2. Running the Tool

  1. Open your microscopy image stack in Fiji.
  2. Run the plugin by navigating to Plugins > Lanczos Resizer.
  3. A dialogue window will appear:
    • Scale Factor: Input your desired scale (e.g., 2.0 to double the size, 0.5 to halve it).
    • Output Bit Depth: Choose 16-bit (recommended) or 8-bit (ideal for publication/presentations).
  4. Click OK.

3. Verifying Results & Scale Bars

  • Go to Analyze > Tools > Scale Bar....
  • The scale bar text size will scale naturally with your new resolution.
  • Press i on your keyboard to view the automated log confirming the math.

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