“The starfile should automatically display. Fill in the rlnDefocus column with the approximate defocus in Å at 0° tilt for each tomogram.”
Hi,
Sorry to bother you. I have a question about how the rlnDefocus value should be determined for IsoNet2.
If the tomograms are processed using a Warp-based workflow, different stages may have their own defocus values, for example from the frame series, tilt series, or the aretomo_alignz stage.
When preparing the STAR file for IsoNet2, should rlnDefocus be taken from the upstream/original 0° defocus value, or should it be taken from the defocus value associated with the later stage that is closest to the final reconstructed tomogram? In other words, for IsoNet2, which defocus value is expected in rlnDefocus: the initial 0° defocus estimated early in the workflow, or the defocus value after tilt-series alignment/reconstruction-related processing?
Thank you!
“The starfile should automatically display. Fill in the rlnDefocus column with the approximate defocus in Å at 0° tilt for each tomogram.”
Hi,
Sorry to bother you. I have a question about how the rlnDefocus value should be determined for IsoNet2.
If the tomograms are processed using a Warp-based workflow, different stages may have their own defocus values, for example from the frame series, tilt series, or the aretomo_alignz stage.
When preparing the STAR file for IsoNet2, should rlnDefocus be taken from the upstream/original 0° defocus value, or should it be taken from the defocus value associated with the later stage that is closest to the final reconstructed tomogram? In other words, for IsoNet2, which defocus value is expected in rlnDefocus: the initial 0° defocus estimated early in the workflow, or the defocus value after tilt-series alignment/reconstruction-related processing?
Thank you!