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Hi @cying111, I was wondering if for your PCR cDNA samples, if you performed UMI ligation for library preparation. If so, did you end up deduplicating read s in your FASTQ/ bam files?
Hi @cying111, I was wondering if for your PCR cDNA samples, if you performed UMI ligation for library preparation. If so, did you end up deduplicating read s in your FASTQ/ bam files?
Thanks,
Sowmya