Hi,
I cant seem to find any pacbio isoseq data (ideally bam) from recent (yesterday!) paper "A systematic benchmark of Nanopore long-read RNA sequencing for transcript-level analysis in human cell lines."
If you could point me to it that would be great!
Joe.
I was looking here: s3://sg-nex-data/data/sequencing_data_ont/bam/genome
and:
They do not appear in here: https://github.com/GoekeLab/sg-nex-data/blob/master/docs/samples.tsv
(no replicate7?)
From supp data data I see, they are labelled as replicate7?:
SGNex_MCF7_PacBio-SMRTcell_replicate5_run1
SGNex_HepG2_PacBio-SMRTcell_replicate7_run1
SGNex_A549_PacBio-SMRTcell_replicate7_run1
SGNex_Hct116_PacBio-SMRTcell_replicate7_run1
SGNex_K562_PacBio-SMRTcell_replicate7_run1
SGNex_HEYA8_PacBio-SMRTcell_replicate4_run1
But in AWS there are no matching replicate / run files: (it would be nice to keep the full name as in the suppdata)
aws s3 ls --no-sign-request s3://sg-nex-data/data/sequencing_data_ont/bam/genome/
PRE SGNex_A549_cDNAStranded_replicate3_run3/
PRE SGNex_A549_cDNAStranded_replicate5_run2/
PRE SGNex_A549_cDNA_replicate1_run2/
PRE SGNex_A549_directRNA_replicate1_run1/
PRE SGNex_A549_directRNA_replicate4_run1/
PRE SGNex_A549_directRNA_replicate5_run1/
PRE SGNex_A549_directRNA_replicate6_run1/
:
:
(truncated output)
Hi,
I cant seem to find any pacbio isoseq data (ideally bam) from recent (yesterday!) paper "A systematic benchmark of Nanopore long-read RNA sequencing for transcript-level analysis in human cell lines."
If you could point me to it that would be great!
Joe.
I was looking here: s3://sg-nex-data/data/sequencing_data_ont/bam/genome
and:
They do not appear in here: https://github.com/GoekeLab/sg-nex-data/blob/master/docs/samples.tsv
(no replicate7?)
From supp data data I see, they are labelled as replicate7?:
SGNex_MCF7_PacBio-SMRTcell_replicate5_run1
SGNex_HepG2_PacBio-SMRTcell_replicate7_run1
SGNex_A549_PacBio-SMRTcell_replicate7_run1
SGNex_Hct116_PacBio-SMRTcell_replicate7_run1
SGNex_K562_PacBio-SMRTcell_replicate7_run1
SGNex_HEYA8_PacBio-SMRTcell_replicate4_run1
But in AWS there are no matching replicate / run files: (it would be nice to keep the full name as in the suppdata)
aws s3 ls --no-sign-request s3://sg-nex-data/data/sequencing_data_ont/bam/genome/
PRE SGNex_A549_cDNAStranded_replicate3_run3/
PRE SGNex_A549_cDNAStranded_replicate5_run2/
PRE SGNex_A549_cDNA_replicate1_run2/
PRE SGNex_A549_directRNA_replicate1_run1/
PRE SGNex_A549_directRNA_replicate4_run1/
PRE SGNex_A549_directRNA_replicate5_run1/
PRE SGNex_A549_directRNA_replicate6_run1/
:
:
(truncated output)