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<!DOCTYPE HTML>
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<a href="index.html" class="logo"><strong>LFCT</strong> - Low Frame-rate Cell Tracking dataset</a>
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<li><a href="https://x.com/IuricichF" class="icon brands fa-twitter"><span class="label">Twitter</span></a></li>
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<header class="major">
<h2>Cell line - MCF10A</h2>
</header>
<p>MCF10A is a non-transformed epithelial cell line. Images for this cell line have been generated with a BioTek Cytation5 under environmental control (37 ℃ and 5% CO<sub>2</sub>) in one field of view. The cells were maintained in a BioTek Biospa8 automated incubator (37 ℃ and 5% CO<sub>2</sub>) during the interval period of imaging. All images have the nuclear reporter H2b-mRuby.</p>
<p>The cell line was seeded onto 12-well plates (Corning Falcon #353043). The seeding density was 2-3×10<sup>4</sup> cells/well. Cells were incubated for 16 hours at 37 ℃ and 5% CO<sub>2</sub>, then the medium was changed to remove dead cells and further incubated for 2 hours. Images were acquired at 4X, 10X, and 20X magnifications with the RFP channel (531/593) to visualize red fluorescent protein, mRuby, as well as the phase contrast channel with 10-15 minute intervals for three days.</p>
<p>The MCF10A cells H2b-mRuby were cultured in DMEM/F12 (Gibco #11330-032) supplemented with 5% horse serum (Gibco #16050122), 20 ng/mL EGF (PeproTech #AF100-15), 0.5 mg/mL Hydrocortisone (Sigma #H0888), 100 ng/mL cholera toxin (Sigma #C8052), and 10 µg/mL insulin (Sigma #I-1882) containing hygromycin (Millipore Sigma #H3274-100MG) (1.5 µg/mL) and puromycin (STEMCELL #73342) (0.1 µg/mL). HEK293T cells expressing H2b-mRuby were cultured in DMEM (Gibco #10313021) supplemented with 10% FBS (V/V) (Gibco #10082139), 2mM L-glutamine (Corning #25-005-CI) containing 0.5 µg/mL puromycin (STEMCELL #73342). For trypsinization, 0.05% trypsin, 0.53 mM EDTA (Corning #25-052-CI) was used.
<div class="posts">
<article>
<a href="#" class="image"><img src="images/FLD_3_mcf10a_10_pc.tif" alt="" /></a>
<h3>FLD_3</h3>
<a href="https://clemson.box.com/s/0wmgo943ppw3yq22r5ux9i590pggh21u">Phase contrast</a> |
<a href="https://clemson.box.com/s/yldtn2vebo9gzbwonwguw27majf5jwfy">Fluorescent channel</a> |
<a href="https://clemson.box.com/s/mam8j8i0tdxpy2wz4e9n8mra2pjw1pyt">Annotations</a> |
<a href="https://clemson.box.com/s/v0ljv6cd8b876kwrjaiffbhsg0kjkxns">Video sample</a>
</article>
<article>
<a href="#" class="image"><img src="images/FLD_4_mcf10a_10_pc.tif" alt="" /></a>
<h3>FLD_4</h3>
<a href="https://clemson.box.com/s/028ngsr5bxgmo2gofh8i20ed4v6gkey8">Phase contrast</a> |
<a href="https://clemson.box.com/s/7seo0xfx9widko65dfe8hr5we069u8pp">Fluorescent channel</a> |
<a href="https://clemson.box.com/s/o4r28ogrpfzhhi9a9yjgtm5e6766qej1">Annotations</a> |
<a href="https://clemson.box.com/s/nnum61wrqzfdexpz47t33b24lkoxnebw">Video sample</a>
</article>
<article>
<a href="#" class="image"><img src="images/FLD_5_mcf10a_10_pc.tif" alt="" /></a>
<h3>FLD_5</h3>
<a href="https://clemson.box.com/s/auxhmzvgra9f3zo1wo7gim8t6066vb0a">Phase contrast</a> |
<a href="https://clemson.box.com/s/ghe1agq86iqa8fldsbu3es7kk4sxtfyp">Fluorescent channel</a> |
<a href="https://clemson.box.com/s/0aptsd16ia9s795gk7x4e98gblm0rdve">Annotations</a> |
<a href="https://clemson.box.com/s/bvfdaqr2oqpqq351t2ghkufjhvtvs2lv">Video sample</a>
</article>
<article>
<a href="#" class="image"><img src="images/FLD_6_mcf10a_10_pc.tif" alt="" /></a>
<h3>FLD_6</h3>
<a href="https://clemson.box.com/s/b7q2jx5f2370b1z7xhnyvlqhm8sq3daw">Phase contrast</a> |
<a href="https://clemson.box.com/s/qtrr6jzmzil8hg6pk5wev0o6ligsjlwt">Fluorescent channel</a> |
<a href="#">Annotations</a> |
<a href="https://clemson.box.com/s/tauoth94ub1jpolubg5g88ew14dnu2hb">Video sample</a>
</article>
<article>
<a href="#" class="image"><img src="images/FLD_7_mcf10a_10_pc.tif" alt="" /></a>
<h3>FLD_7</h3>
<a href="https://clemson.box.com/s/val41npyiqbk1aejfh7zqa399912t4uf">Phase contrast</a> |
<a href="https://clemson.box.com/s/vru6dnok298k8r55zr8ef8kdk5ugdxx2">Fluorescent channel</a> |
<a href="https://clemson.box.com/s/twr2f69d8e7bchs0g4ly1438t7df29g1">Annotations</a> |
<a href="https://clemson.box.com/s/soa38e8rg11fh3qxtv4fkpesg4b5bim8">Video sample</a>
</article>
<article>
<a href="#" class="image"><img src="images/FLD_8_mcf10a_10_pc.tif" alt="" /></a>
<h3>FLD_8</h3>
<a href="https://clemson.box.com/s/sz1vb36ng3aij25mbcqi2fi3mayu1spd">Phase contrast</a> |
<a href="https://clemson.box.com/s/kzw4fb92w8i2jfpopd6ev1kzqn27xfbp">Fluorescent channel</a> |
<a href="https://clemson.box.com/s/wseo1gdi8l5ied87x2nk7b69jjze0vyr">Annotations</a> |
<a href="https://clemson.box.com/s/cbnngi29ge4zmmwuod0q7lwpxmkz96fr">Video sample</a>
</article>
<article>
<a href="#" class="image"><img src="images/FLD_9_mcf10a_10_pc.tif" alt="" /></a>
<h3>FLD_9</h3>
<a href="https://clemson.box.com/s/qy5ufp82uvomvtszhb5tz3rkuel2uyn3">Phase contrast</a> |
<a href="https://clemson.box.com/s/ezji8nsk0vlbgs12kvuyrwoeli4b1330">Fluorescent channel</a> |
<a href="#">Annotations</a> |
<a href="https://clemson.box.com/s/m9yu68r6m0twlw1b2ef0h2pddzvcjkjc">Video sample</a>
</article>
<article>
<a href="#" class="image"><img src="images/FLD_10_mcf10a_10_pc.tif" alt="" /></a>
<h3>FLD_10</h3>
<a href="https://clemson.box.com/s/kjaw7bprlw0yf6zfy8v9w19afard8syh">Phase contrast</a> |
<a href="https://clemson.box.com/s/nrvt4a5hex6s0ph20rymje6h3sqrryg3">Fluorescent channel</a> |
<a href="https://clemson.box.com/s/twr2f69d8e7bchs0g4ly1438t7df29g1">Annotations</a> |
<a href="https://clemson.box.com/s/svazc6kby22lpmaylmk1y3deqxyt5yo8">Video sample</a>
</article>
<article>
<a href="#" class="image"><img src="images/FLD_11_mcf10a_10_pc.tif" alt="" /></a>
<h3>FLD_11</h3>
<a href="https://clemson.box.com/s/q4grpabidv51v0qsgd7lqcv8nil8a1su">Phase contrast</a> |
<a href="https://clemson.box.com/s/zxeu8hvhmhsccgtadzarwnnp1gtiyqey">Fluorescent channel</a> |
<a href="https://clemson.box.com/s/wseo1gdi8l5ied87x2nk7b69jjze0vyr">Annotations</a> |
<a href="https://clemson.box.com/s/a7ftvfunuelwrgzgy32j79hlcwvd4zda">Video sample</a>
</article>
<article>
<a href="#" class="image"><img src="images/FLD_12_mcf10a_10_pc.tif" alt="" /></a>
<h3>FLD_12</h3>
<a href="https://clemson.box.com/s/c4y15i01x1i67isydoyioseocc4r0kn6">Phase contrast</a> |
<a href="https://clemson.box.com/s/iepqth2n163ujnrg5r1bptoy4l7x3749">Fluorescent channel</a> |
<a href="#">Annotations</a> |
<a href="https://clemson.box.com/s/e5aq5zwz6bvtal9vd8d0x1zsob24gkb9">Video sample</a>
</article>
</div>
</section>
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<h2>Contacts</h2>
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<li class="icon solid fa-envelope"><a href="#">fiurici@clemson.edu</a></li>
<li class="icon solid fa-home">821 McMillan Rd <br />
Clemson, SC 29631</li>
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