Rarely outputs of correct_barcode_nano.py

[Ckenen]

I am no sure there is any problem in correct_barcode_nano.py LINE70 and LINE81-LINE92?

LINE70 will over estimate the pass reads. Because 1 read will be counting twice or more when it is similary (distance <= 4) to multiple whitelist barcode.

LINE81-LINE92 will resulting the discard of expected pass reads. The output is indeed little.

[Acribbs]

We realised subsequently that correcting for a distance of 4 is not recommended because of barcode clashing issues. We recommend 2 or less distance now.

FYI in our latest version of our beads we no longer use a dimer barcode as the ONT chemistry is now substantially accurate to perform whitelisting without dimers.